Choline or CDP-choline restores hypotension and improves myocardial and respiratory functions in dogs with experimentally – Induced endotoxic shock. (December 2021)

• Record Type: Journal Article
• Authors: Kocaturk, Meric; Yilmaz, Zeki; Cansev, Mehmet; Ozarda, Yesim; Ceron, Jose Joaquin; Buturak, Ali; Ulus, Ismail H.
• Is Part Of: Research in veterinary science. Volume 141(2021)
• Page Start: 116
• Page End: 128
• Publication Date: 2021-12
• Subjects: Choline -- Endotoxemia -- Sepsis -- Cardiopulmonary function -- Dogs; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2021.10.010
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Endotoxin shock is associated with severe impairments in cardiovascular and respiratory functions. We showed previously that choline or cytidine-5′-diphosphocholine (CDP-choline) provides beneficial effects in experimental endotoxin shock in dogs. The objective of the present study was to determine the effects of choline or CDP-choline on endotoxin-induced cardiovascular and respiratory dysfunctions. Dogs were treated intravenously (i.v.) with saline or endotoxin (LPS, 0.1 mg/kg) 5 min before i.v. infusion of saline, choline (20 mg/kg) or CDP-choline (70 mg/kg). Blood pressure, cardiac rate, myocardial and left ventricular functions, respiratory rate, blood gases, serum electrolytes and cardiac injury markers were determined before and at 0.5-48 h after endotoxin. Plasma tumor necrosis factor alpha (TNF-α), high mobility group box-1 (HMGB1), catecholamine and nitric oxide (NO) levels were measured 2 h and 24 h after the treatments. Endotoxin caused immediate and sustained reductions in blood pressure, cardiac output, pO2 and pH; changes in left ventricular functions, structure and volume parameters; and elevations in heart rate, respiratory rate, pCO2 and serum electrolytes (Na, K, Cl, Ca and P). Endotoxin also resulted in elevations in blood levels of cardiac injury markers, TNF-α, HMGB1, catecholamine and NO. In choline- or CDP-choline-treated dogs, all endotoxin effects were much smaller in magnitude and shorter in duration than observed values in controls. These data show that treatment with choline or CDP-choline improves functions of cardiovascular and respiratory systems in experimental endotoxemia and suggest that they may be useful in treatment of endotoxin shock in clinical setting. Highlights: Endotoxin shock associates with severe impairment in cardiovascular and respiratory functions. Choline or cytidine-5'-diphosphocholine (CDP-choline) provides beneficial effects in experimental endotoxemia in dogs. Choline or CDP-choline attenuates endotoxin-induced alterations in cardiovascular and respiratory functions in dogs with experimental endotoxemia.