A BODIPY‐Based Far‐Red‐Absorbing Fluorescent Probe for Hypochlorous Acid Imaging. Issue 4 (12th January 2022)

• Record Type: Journal Article
• Authors: Wan, Zhaoxiong; Yu, Shupei; Wang, Qi; Tobia, John; Chen, Hao; Li, Zhanjun; Liu, Xuan; Zhang, Yuanwei
• Is Part Of: ChemPhotoChem. Volume 6:Issue 4(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-01-12
• Subjects: BODIPY -- cell imaging -- hypochlorous acid -- ratiometric sensing -- reactive oxygen species; Photochemistry -- Periodicals; Periodicals; Electronic journals; 541.35
• DOI: 10.1002/cptc.202100250
• Languages: English
• ISSNs: 2367-0932

Abstract

Abstract: Hypochlorous acid (HClO) is produced by white blood cells to defend against injury and bacteria. However, as one of the reactive oxygen species, high intracellular HClO concentration could lead to chronic diseases that affect the cardiovascular and nervous systems. To monitor HClO concentrations in bio‐samples, the fluorescent probe is preferred to have: a) Absorbability in the far‐red window with reduced light toxicity and improved tissue penetration depth; b) Ratiometric features for accurate analysis. In this study, we reported a far‐red ratiometric HClO fluorescence probe based on BODIPY chromophore and aldoxime sensing group. Not only the color change of the probe solution can be detected by the naked eye, but also the emission ratios (I645 /I670 ) showed a significant increase upon the introduction of HClO. More importantly, the feasibility of HClO monitoring in biosamples was demonstrated in vitro using a confocal microscope. Abstract : Ratiometric mapping of intracellular HClO concentration is reported using a BODIPY‐based, far‐red‐absorbing fluorescent sensor. This sensor consists of two parts, firstly an aldoxime group that can interact with HClO, and the second is a far‐red‐absorbing BODIPY chromophore as a fluorescent reporter.