Reversibly Photoswitching Upconversion Nanoparticles for Super‐Sensitive Photoacoustic Molecular Imaging. (25th February 2022)

• Record Type: Journal Article
• Authors: Liu, Cheng; Zheng, Xianchuang; Dai, Tingting; Wang, Huiliang; Chen, Xian; Chen, Bing; Sun, Tianying; Wang, Feng; Chu, Steven; Rao, Jianghong
• Is Part Of: Angewandte Chemie. Volume 134:Number 19(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-25
• Subjects: Imaging Agents -- Near-infrared -- Photoacoustic Imaging -- Reversible Photoswitching -- Upconversion Nanoparticles; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202116802
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Photoacoustic (PA) imaging uses light excitation to generate the acoustic signal for detection and improves tissue penetration depth and spatial resolution in the clinically relevant depth of living subjects. However, strong background signals from blood and pigments have significantly compromised the sensitivity of PA imaging with exogenous contrast agents. Here we report a nanoparticle‐based probe design that uses light to reversibly modulate the PA emission to enable photoacoustic photoswitching imaging (PAPSI) in living mice. Such a nanoprobe is built with upconverting nanocrystals and photoswitchable small molecules and can be switched on by NIR light through upconversion to UV energy. Reversibly photoswitching of the nanoprobe reliably removed strong tissue background, increased the contrast‐to‐noise ratio, and thus improved imaging sensitivity. We have shown that PAPSI can image 0.05 nM of the nanoprobe in hemoglobin solutions and 10 4 labeled cancer cells after implantation in living mice using a commercial PA imager. Abstract : An upconverting nanoparticle probe can be switched on and off by near‐infrared (NIR) light for multiple cycles of photoacoustic imaging (PA). Through reversible modulation of the probe PA activation state, a background‐free PA signal can be recovered in imaging 0.05 nM of the probe in hemoglobin (Hb) solution and 10 4 labeled cancer cells subcutaneously implanted in mice.