Human toxoplasmosis: a comparative evaluation of the diagnostic potential of recombinantToxoplasma gondiiROP5 and ROP18 antigens. Issue 10 (October 2015)

• Record Type: Journal Article
• Authors: Grzybowski, Marcin M.; Gatkowska, Justyna M.; Dziadek, Bożena; Dzitko, Katarzyna; Długońska, Henryka
• Is Part Of: Journal of medical microbiology. Volume 64:Issue 10(2015)
• Publication Date: 2015-10
• Subjects: Medical microbiology -- Periodicals; 616.9041
• DOI: 10.1099/jmm.0.000148
• Languages: English
• ISSNs: 0022-2615

Abstract

Abstract : N/A: Toxoplasmosis is one of the most common parasitic diseases worldwide and it poses a serious challenge regarding prevention, diagnosis and therapy. The commonly used diagnostic methods are mostly based on the detection of specific antibodies in sera. Since they are not always accurate enough and do not allow precise definition of the phase of the Toxoplasma gondii infection, there is an urgent need to find specific molecular markers of acute or chronic infection stages. This study provides a comparative assessment of recombinant ROP5 and ROP18 T. gondii proteins in the serodiagnosis of human toxoplasmosis. We found that both ROP5 and ROP18 proteins allowed the detection of specific IgM and IgG antibodies with a relatively low sensitivity; however, ROP18 IgM ELISA proved to be more sensitive than the SAG1 assay. This study also points to a relatively weak potential of the corresponding native ROP5 and ROP18 kinases in the generation of a strong antibody response in humans.