ATPase subunits of the 26S proteasome are important for oocyte maturation in the brown planthopper. Issue 3 (5th February 2022)

• Record Type: Journal Article
• Authors: Cheng, Xu; Wang, Wei; Zhang, Lu; Yang, Rui‐Rui; Ma, Ya; Bao, Yan‐Yuan
• Is Part Of: Insect molecular biology. Volume 31:Issue 3(2022)
• Page Start: 317
• Page End: 333
• Publication Date: 2022-02-05
• Subjects: ATPase regulatory subunit -- Nilaparvata lugens -- oocyte maturation -- proteasome activity -- transcription regulation; Insects -- Molecular aspects -- Periodicals; 595.7
• DOI: 10.1111/imb.12761
• Languages: English
• ISSNs: 0962-1075

Abstract

Abstract: The 26S proteasome is the major engine of protein degradation in all eukaryotic cells. Adenosine triphosphatase (ATPase) regulatory subunits (Rpts) are constituents of the proteasome that are involved in the unfolding and translocation of substrate proteins into the core particle. In this study, by using the brown planthopper Nilaparvata lugens as a model insect, we report the biological importance of Rpts in female reproduction. We identified six homologous Rpt genes ( Rpt1 ‐ 6 ) in N. lugens . These genes were detected at high transcript levels in eggs and ovaries of females but at low transcript levels in males. RNA interference‐mediated knockdown of N. lugens Rpt genes significantly decreased the proteolytic activity of the proteasome and impeded the transcription of triacylglycerol lipase and vitellogenin genes in the fat bodies and ovaries of adult females and reduced the triglyceride content in the ovaries. The decrease in the proteolytic activity of the proteasome via knockdown of Rpt s also downregulated the transcription of the CYP307A2 gene encoding an important rate‐limiting enzyme in the 20‐hydroxyecdysone biosynthetic pathway in the ovaries, reduced 20E production in adult females and impaired ovarian development and oocyte maturation, leading to the failure of egg production and egg‐laying. These novel findings indicate that Rpts are required for the proteolytic activity of the proteasome, which is important for female reproductive success in N. lugens . Abstract : Schematic diagram of Rpt in female reproduction in Nilaparvata lugens. The knockdown of Rpts decreased the proteolytic activity of the proteasome in N. lugens and impeded the transcription of triacylglycerol lipase and Vg genes in fat bodies and ovaries. Rpts were also related to the expression of CYP307A2, a key rate‐limiting enzyme gene in the 20E biosynthetic pathway, in female ovaries. The knockdown of Rpts disrupted triacylglycerol metabolism in the fat bodies and oocytes, affected Vg utilization in the developing oocytes and inhibited 20E synthesis in the ovaries, thereby leading to a failure of oocyte maturation and development.