Serial macromolecular crystallography at ALBA Synchrotron Light Source. (5th April 2022)

• Record Type: Journal Article
• Authors: Martin-Garcia, Jose M.; Botha, Sabine; Hu, Hao; Jernigan, Rebecca; Castellví, Albert; Lisova, Stella; Gil, Fernando; Calisto, Barbara; Crespo, Isidro; Roy-Chowdhury, Shatabdi; Grieco, Alice; Ketawala, Gihan; Weierstall, Uwe; Spence, John; Fromme, Petra; Zatsepin, Nadia; Boer, Dirk Roeland; Carpena, Xavi
• Is Part Of: Journal of synchrotron radiation. Volume 29:Part 3(2022)
• Page Start: 896
• Page End: 907
• Publication Date: 2022-04-05
• Subjects: serial synchrotron crystallography -- viscous jet -- LCP -- microcrystal -- ALBA -- XALOC; Synchrotron radiation -- Periodicals; Free electron lasers -- Periodicals; 539.73505
• DOI: 10.1107/S1600577522002508
• Languages: English
• ISSNs: 0909-0495

Abstract

Abstract : We report the first SSX experiments with viscous jets conducted at ALBA beamline BL13‐XALOC. We confirm that the current capabilities at BL13‐XALOC enable atomic resolution determination of protein structures from microcrystals using viscous jets at room temperature. Abstract : The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13‐XALOC. Small crystals (15–30 µm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α‐spectrin‐SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X‐ray beam with a high‐viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high‐resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 µm using viscous jets at room temperature. Thus, BL13‐XALOC can provide a feasible alternative to X‐ray free‐electron lasers when determining snapshots of macromolecular structures.