Isolation of ocotillol/ocotillone from Fouquieria splendens (Ocote) using a batch reactor. Issue 5 (2nd May 2022)

• Record Type: Journal Article
• Authors: Morales-Cepeda, Ana Beatriz; Macclesh del Pino-Pérez, Luis Alejandro; Marmolejo, Mireya; Rivera-Armenta, José Luis; Peraza-Vázquez, Hernan
• Is Part Of: Preparative biochemistry & biotechnology. Volume 52:Issue 5(2022)
• Page Start: 540
• Page End: 548
• Publication Date: 2022-05-02
• Subjects: Extraction -- isolation -- ocotillol/ocotillone -- sustainable production; Biochemistry -- Technique -- Periodicals; Biotechnology -- Technique -- Periodicals; Biochemistry -- methods -- Periodicals; Biotechnology -- methods -- Periodicals; 660.6
• DOI: 10.1080/10826068.2021.1972425
• Languages: English
• ISSNs: 1082-6068

Abstract

Abstract: Exploitation of the "ocotillo" tree ( Fouquieria splendens Engelm) to produce ocotillol/ocotillone (o/o) in Northern Mexico hasn't been explored to this date. In the present work, isolation of ocotillol/ocotillone was performed through a batch reactor, which allowed the elimination of alcohol and phenols present in the ocote plant, yielding only resins and waxes from the ocote trunks. The ocotillol/ocotillone was extracted from the remaining resin on the ocote logs by solvent extraction in a batch reactor, to be crystallized after its extraction using a rotary evaporator. FTIR, and NMR analysis exhibited the characteristic ginsenoside bands, while the UV-vis spectrum of ocotillol/ocotillone depicted an absorption band belonging to the O–H bonds, indicating that the group is anchored to the ginsenoside structure and not due to a water signal. Lastly, the thermogravimetric analysis described a common behavior among other ginsenosides. Production of ocotillo/ocotillone has an energy consumption of 3624 kWh/gr. With a cost of $0.478 USD in laboratory equipment, which translates as a plausible sustainable production of ocotillol/ocotillone