A smart mitochondria-targeting TP-NIR fluorescent probe for the selective and sensitive sensing of H2S in living cells and mice. (12th April 2021)

• Record Type: Journal Article
• Authors: Yang, Qiaomei; Zhou, Liyi; Peng, Longpeng; Yuan, Gangqiang; Ding, Haiyuan; Tan, Libin; Zhou, Yizhuang
• Is Part Of: New journal of chemistry. Volume 45:Number 16(2021)
• Page Start: 7315
• Page End: 7320
• Publication Date: 2021-04-12
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/d1nj00840d
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : Hydrogen sulfide (H2 S) is one of the important gaseous signalling molecules, which plays key roles in various critical biological processes. Abstract : Hydrogen sulfide (H2 S) is one of the important gaseous signalling molecules, which plays key roles in various critical biological processes. In this work, we report a novel two-photon near-infrared (TP-NIR) fluorescent probe (MNIR-H2 S ) for the sensing of mitochondrial H2 S in living cells, tissues, zebrafish and nude mice. Using a xanthene dye as a TP-excitation (810 nm) NIR-emission (668 nm) fluorophore, a well-known H2 S response moiety with a robust intramolecular charger transfer (ICT) effect can quickly respond to H2 S and effectively quench the fluorescence intensity, and an oxonium cation structure serves as a mitochondria-targeting site. Notably, MNIR-H2 S displays high sensitivity, high selectivity, low cytotoxicity, powerful mitochondrial localization ability, deep tissue penetration, and a very minimal imaging background. Biological imaging results show the biological application of MNIR-H2 S as a robust new tool for use in H2 S imaging in biological systems.