Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection. Issue 17 (4th April 2022)
- Record Type:
- Journal Article
- Title:
- Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection. Issue 17 (4th April 2022)
- Main Title:
- Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection
- Authors:
- Zhao, Rujian
Yu, Chunxu
Lu, Baiyang
Li, Bingling - Abstract:
- Abstract : We report a universal and signal-on HCR based detection platform via coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA, with practical sensitivity as low as ∼aM. Abstract : We report a universal and signal-on HCR based detection platform via innovatively coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA. The CRISPR-HCR platform coupling with an upstream amplifier can achieve a practical sensitivity as low as ∼aM of ASFV gene in serum.
- Is Part Of:
- RSC advances. Volume 12:Issue 17(2022)
- Journal:
- RSC advances
- Issue:
- Volume 12:Issue 17(2022)
- Issue Display:
- Volume 12, Issue 17 (2022)
- Year:
- 2022
- Volume:
- 12
- Issue:
- 17
- Issue Sort Value:
- 2022-0012-0017-0000
- Page Start:
- 10374
- Page End:
- 10378
- Publication Date:
- 2022-04-04
- Subjects:
- Chemistry -- Periodicals
540.5 - Journal URLs:
- http://pubs.rsc.org/en/Journals/JournalIssues/RA ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/d2ra01332k ↗
- Languages:
- English
- ISSNs:
- 2046-2069
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 8036.750300
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 21415.xml