CdTe@CdS quantum dots for labeling and imaging macrophages in liver frozen sections below the freezing point. Issue 15 (22nd March 2022)

• Record Type: Journal Article
• Authors: He, Yan-Fei; An, Chang-Zhi; Hou, Xiao-Lin; Zhong, Zi-Tao; Li, Chao-Qing; Chen, Wei; Liu, Bo; Zhao, Yuan-Di
• Is Part Of: Journal of materials chemistry. Volume 10:Issue 15(2022)
• Page Start: 2952
• Page End: 2962
• Publication Date: 2022-03-22
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d1tb02781f
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : CdTe@CdS core–shell quantum dots with different particle sizes are synthesized by an aqueous method, and coating them with a CdS shell layer improves the quantum yield (36% → 59%) and fluorescence stability (37% → 77%) of CdTe@CdS quantum dots. Abstract : CdTe@CdS core–shell quantum dots with different particle sizes are synthesized by an aqueous method, and coating them with a CdS shell layer improves the quantum yield (36% → 59%) and fluorescence stability (37% → 77%) of CdTe@CdS quantum dots. When the KCl concentration (mass fraction) in the system is 15%, the CdTe@CdS quantum dot dispersion system remains in the liquid state at −20 °C, and the low temperature increases the fluorescence intensity. A QD–Ab probe is obtained after CdTe@CdS quantum dots are coupled with IgG; the circular dichroism shows that the IgG protein structure is not destroyed, while capillary electrophoresis, agarose gel electrophoresis and flow cytometry verify the conjugation efficiency. With rabbit anti-mouse EMR1 antibody as the primary antibody and QD–Ab as the secondary antibody, the hepatic macrophages in liver frozen sections are fluorescently labeled at −20 °C, and it is found that they are radially distributed in hepatic sinusoids with specific and highly efficient labeling; these results are verified by H&E staining and TEM. This technology can provide important technical support for in-depth understanding of the distribution of liver immune cells in the liver, and it can further provide a scientific basis to understand the relationship between the liver structure and function and pathological changes.