Biomimetic self-assembly of lipase-zeolitic imidazolate frameworks with enhanced biosensing of protox inhibiting herbicides. Issue 42 (18th October 2021)

• Record Type: Journal Article
• Authors: Cheng, Chuanchuan; Shen, Cai; Lai, Oi-Ming; Tan, Chin-Ping; Cheong, Ling-Zhi
• Is Part Of: Analytical methods. Volume 13:Issue 42(2021)
• Page Start: 4974
• Page End: 4984
• Publication Date: 2021-10-18
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d1ay01307f
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Protox inhibiting herbicides such as nitrofen have detrimental effects on the environment and human health. Abstract : Protox inhibiting herbicides such as nitrofen have detrimental effects on the environment and human health. The current work aims to fabricate a Candida rugosa lipase (CRL)-based electrochemical sensor for rapid and sensitive detection of protox inhibiting herbicides (nitrofen). We proposed the use of poly(vinylpyrrolidone) (PVP) and amino-acids to promote accumulation of Zn 2+ ions at the surfaces of Candida rugosa lipase (CRL) and subsequently induce self-assembly of a CRL-zeolitic imidazolate framework (ZIF) structure. This process can be easily and rapidly achieved via a one-pot facile self-assembly method. Steady-state fluorescence spectroscopy indicated that CRL has undergone a conformational change following encapsulation within the ZIF structure. This conformational change is beneficial as the prepared PVP/Glu/CRL@ZIF-8 exhibited enhanced catalytic activity (207% of native CRL), and higher substrate affinity (lower K m than native CRL) and showed high stability under harsh denaturing conditions. PVP/Glu/CRL@ZIF-8 was finally used for electrochemical biosensing of nitrofen. The fabricated biosensor has a wide linear detection range (0–100 μM), a lower limit of detection and a good recovery rate.