Identification of C-type isolectins in the venom of the scorpionfish Scorpaena plumieri. (1st March 2015)

• Record Type: Journal Article
• Authors: Andrich, F.; Richardson, M.; Naumann, G.B.; Cordeiro, M.N.; Santos, A.V.; Santos, D.M.; Oliveira, J.S.; de Lima, M.E.; Figueiredo, S.G.
• Is Part Of: Toxicon. Volume 95(2015)
• Page Start: 67
• Page End: 71
• Publication Date: 2015-03-01
• Subjects: Fish venom -- Scorpaena plumieri -- Scorpionfish -- C-type lectin -- Hemagglutination -- Isolectins; SpV Scorpaena plumieri Venom -- FV Fraction V with hemagglutinating activity -- SDS-PAGE Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis -- MALDI-TOF Matrix Assisted Laser Desorption Ionization Time-Of-Flight -- MS Mass Spectrometry -- RP-HPLC Reversed Phase High Performance Liquid Chromatography -- TFA Trifluoroacetic Acid -- ACN Acetonitrile -- CTLD C-Type Lectin-like Domain -- CRD Carbohydrate Recognition Domain -- Sp-CL S. plumieri C-type Lectin; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2015.01.004
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Chemical analyses of the hemagglutinating fraction from Scorpaena plumieri venom revealed that it contains five components (Sp-CL 1-5) with similar chromatographic elution profiles (35–38% of acetonitrile), molecular masses (16, 800–17, 000 Da) and N-terminal sequences, suggesting that they are isoforms of the same protein. The amino acid sequence of Sp-CL4 was determined and shown to have homology with fish C-type lectins. These data demonstrate for the first time the presence of C-type isolectins in a scorpionfish venom. Highlights: A hemagglutinating fraction (FV) was purified from Scorpaena plumieri fish venom. FV contains five components with similar molecular masses and N-terminal sequences. These compounds were purified and named Sp-CL 1-5. Sp-CL4 amino acid sequence exhibited homology with galactose-binding C-type lectins. We demonstrate the presence of C-type lectins isoforms in scorpionfish venom.