Reconciling Egg- and Antigen-Based Estimates of Schistosoma mansoni Clearance and Reinfection: A Modeling Study. (6th August 2021)

• Record Type: Journal Article
• Authors: Clark, Jessica; Moses, Arinaitwe; Nankasi, Andrina; Faust, Christina L; Moses, Adriko; Ajambo, Diana; Besigye, Fred; Atuhaire, Aaron; Wamboko, Aidah; Carruthers, Lauren V; Francoeur, Rachel; Tukahebwa, Edridah M; Prada, Joaquin M; Lamberton, Poppy H L
• Is Part Of: Clinical infectious diseases. Volume 74:Number 9(2022)
• Page Start: 1557
• Page End: 1563
• Publication Date: 2021-08-06
• Subjects: Diagnostics -- temporal dynamics -- POC-CCA -- G-Score -- Kato-Katz; Communicable diseases -- Periodicals; 616.905
• DOI: 10.1093/cid/ciab679
• Languages: English
• ISSNs: 1058-4838

Abstract

Abstract: Background: Despite decades of interventions, 240 million people have schistosomiasis. Infections cannot be directly observed, and egg-based Kato-Katz thick smears lack sensitivity, affected treatment efficacy and reinfection rate estimates. The point-of-care circulating cathodic antigen (referred to from here as POC-CCA+) test is advocated as an improvement on the Kato-Katz method, but improved estimates are limited by ambiguities in the interpretation of trace results. Method: We collected repeated Kato-Katz egg counts from 210 school-aged children and scored POC-CCA tests according to the manufacturer's guidelines (referred to from here as POC-CCA+) and the externally developed G score. We used hidden Markov models parameterized with Kato-Katz; Kato-Katz and POC-CCA+; and Kato-Katz and G-Scores, inferring latent clearance and reinfection probabilities at four timepoints over six-months through a more formal statistical reconciliation of these diagnostics than previously conducted. Our approach required minimal but robust assumptions regarding trace interpretations. Results: Antigen-based models estimated higher infection prevalence across all timepoints compared with the Kato-Katz model, corresponding to lower clearance and higher reinfection estimates. Specifically, pre-treatment prevalence estimates were 85% (Kato-Katz; 95% CI: 79%–92%), 99% (POC-CCA+; 97%–100%) and 98% (G-Score; 95%–100%). Post-treatment, 93% (Kato-Katz; 88%–96%), 72% (POC-CCA+; 64%–79%) and 65% (G-Score; 57%–73%) of those infected were estimated to clear infection. Of those who cleared infection, 35% (Kato-Katz; 27%–42%), 51% (POC-CCA+; 41%–62%) and 44% (G-Score; 33%–55%) were estimated to have been reinfected by 9-weeks. Conclusions: Treatment impact was shorter-lived than Kato-Katz–based estimates alone suggested, with lower clearance and rapid reinfection. At 3 weeks after treatment, longer-term clearance dynamics are captured. At 9 weeks after treatment, reinfection was captured, but failed clearance could not be distinguished from rapid reinfection. Therefore, frequent sampling is required to understand these important epidemiological dynamics. Abstract : The impact of treatment for schistosomiasis in high-endemicity settings is short-lived because treatment is far less efficacious than the World Health Organization–recommended Kato-Katz method would suggest, and reinfection occurs more rapidly than is detected by current surveillance time frames.