Bacterial extracellular polymeric substances as potential saliva substitute. Issue 1 (21st March 2022)

• Record Type: Journal Article
• Authors: Kardas, Piotr; Astasov-Frauenhoffer, Monika; Braissant, Olivier; Bornstein, Michael M; Waltimo, Tuomas
• Is Part Of: FEMS microbiology letters. Volume 369:Issue 1(2022)
• Publication Date: 2022-03-21
• Subjects: xerostomia -- bacteria -- extracellular polymeric substance matrix -- rheology -- lubrication -- biotechnology; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fnac028
• Languages: English
• ISSNs: 0378-1097

Abstract

Abstract: This proof-of-principle study aims to find commensal oral bacteria that can produce extracellular polymeric substances (EPS), which have similar lubrication properties to saliva and could serve as saliva substitutes. Saliva and plaque samples were collected from 21 generally healthy individuals. Primary screening was done by conventional culturing and Gram-staining; all species selected for further analysis were identified by MALDI-TOF and deposited in DSMZ. Lactobacillus gasseri (DSM32453 and DSM32455), Lactobacillus rhamnosus (DSM32452), Lactobacillus paracasei (DSM32454), and Streptococcus sanguinis (DSM32456) produced 413.6, 415.7, 431.1, 426.8, and 877.6 µg/ml of EPS, respectively. At the same time calcium dissolution could not be detected for both L. gasseri strains, minimal dissolution for the other three: S. sanguinis 0.3 mm, and 3.7 mm for L. rhamnosus and L. paracasei . There were no differences found between the EPS samples and the saliva for the effect of shear rate on the viscosity and for the effect of sliding speed on lubrication properties. In conclusion, five commensal bacterial strains have been isolated, all able to produce EPS and lead to no or to low calcium dissolution. EPS produced exhibits rheological and tribological properties comparable to human saliva. A total of four out of five selected strains are probiotic and, therefore, may exhibit additional beneficial influence within the oral cavity. Abstract : Commensal oral bacteria can produce EPS, which have similar lubrication properties to saliva and could serve as saliva substitutes.