Utilization of N, S‐doped carbon dots as a fluorescent nanosensor for determination of cromolyn based on inner filter effect: application to aqueous humour. (28th February 2022)

• Record Type: Journal Article
• Authors: Abd Elhaleem, Shymaa M.; Elsebaei, Fawzi; Shalan, Shereen; Belal, Fathalla
• Is Part Of: Luminescence. Volume 37:Number 5(2022)
• Page Start: 713
• Page End: 721
• Publication Date: 2022-02-28
• Subjects: aqueous humour -- cromolyn sodium -- inner filter effect -- nitrogen/sulfur–carbon quantum dots; Luminescence -- Periodicals; Bioluminescence -- Periodicals; Chemiluminescence -- Periodicals; Luminescence -- Periodicals; 535.35
• DOI: 10.1002/bio.4212
• Languages: English
• ISSNs: 1522-7235

Abstract

Abstract: A simple and eco‐friendly hydrothermal technique is used to prepare water soluble N‐ and S‐co‐doped carbon quantum dots probes (N, S‐CQDs) from thiosemicarbazide and citric acid. Several characterization techniques were performed to ensure the successful synthesis of highly luminescent N, S‐CQDs. The prepared probe exhibited analytical potential as an optical nanosensor for the spectrofluorimetric determination of cromolyn sodium (CRO) in its pharmaceutical dosage forms and aqueous humour. The emission intensity of the synthesized N, S‐CQDs was measured at 411 nm after excitation at 345 nm. Addition of increasing concentrations of CRO to N, S‐CQDs led to quenching of its fluorescence intensity. CRO was investigated within a wide concentration range 10.0–150.0 μM with a limit of detection of 2.0 μM and a limit of quantification of 6.0 μM. The quenching of fluorescent N, S‐CQDs occurred through the inner filter effect (IFE). The developed spectrofluorimetric method was successfully optimized and validated according to the International Council of Harmonisation guidelines (ICH). The method greenness is proved through using both Eco‐Scale and AGREE approaches. Abstract : N, S‐CQDs were used as a fluorescent nanosensor for determination of cromolyn sodium in its dosage forms and aqueous humour. N, S‐CQDs exhibited high fluorescence intensity at 411 nm after excitation at 345 nm. The native fluorescence intensity of the prepared dots was efficiently quenched after the addition of increasing concentrations of CRO.