Three‐dimensional visualization of secondary neurulation in chick embryos using microCT. Issue 5 (1st December 2021)

• Record Type: Journal Article
• Authors: Choi, Sejin; Kim, Kyung Hyun; Kim, Seung‐Ki; Wang, Kyu‐Chang; Lee, Ji Yeoun
• Is Part Of: Developmental dynamics. Volume 251:Issue 5(2022)
• Page Start: 885
• Page End: 896
• Publication Date: 2021-12-01
• Subjects: caudal cell mass -- chick embryo -- microCT -- secondary neurulation; Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.441
• Languages: English
• ISSNs: 1058-8388

Abstract

Abstract: Background: Defects in secondary neurulation play an important role in neural tube defects. Researchers have investigated the processes of secondary neurulation and caudal body formation mainly by microscopic observations and molecular experiments. Although conventional histology is a powerful tool for observing the details of morphology, it has limitations in the presentation of gross three‐dimensional (3D) configurations of small embryos. The goal of this study was to visualize secondary neurulation and related structures in chick embryos in Hamburger and Hamilton (HH) stages 10–22 using microCT. Results: The gross morphology of the chick embryo of various developmental stages was well visualized using microCT. Also, the detailed structures of the caudal cell mass (CCM) were presented starting from HH stage 12 to stage 16. The spatiotemporal relationship of CCM with the floor plate of the neural tube and notochord was shown. The dynamic changes of the chordoneural hinge, the cavitation of the secondary neural tube, and the primitive streak were described throughout the early stages of secondary neurulation. Conclusions: By utilizing the advantages of the microCT technique, our study shed light on the secondary neurulation in early‐stage chick embryos and this can be the 3D reference for related structures. Key Findings: Gross morphology of the chick embryo of various developmental stages was well visualized using microCT. Detailed structures of the caudal cell mass and its spatiotemporal relationship with the floor plate of the neural tube and notochord were presented. Dynamic changes of the chordoneural hinge, the cavitation of the secondary neural tube, and the primitive streak were described throughout the early stages of secondary neurulation.