Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast. Issue 5 (13th October 2021)

• Record Type: Journal Article
• Authors: Pujol‐Carrion, Nuria; Gonzalez‐Alfonso, Alma; Puig, Sergi; de la Torre‐Ruiz, Maria Angeles
• Is Part Of: Microbial biotechnology. Volume 15:Issue 5(2022)
• Page Start: 1525
• Page End: 1541
• Publication Date: 2021-10-13
• Subjects: Microbial biotechnology -- Periodicals; Biotechnology; Microbiology; 660.62
• DOI: 10.1111/1751-7915.13939
• Languages: English
• ISSNs: 1751-7915

Abstract

Summary: Ferritin proteins have an enormous capacity to store iron in cells. In search for the best conditions to accumulate and store bioavailable iron, we made use of a double mutant null for the monothiol glutaredoxins GRX3 and GRX4 . The strain grx3grx4 accumulates high iron concentrations in the cytoplasm, making the metal easily available for ferritin chelation. Here, we perform a comparative study between human (L and H) and soya bean ferritins (H1 and H2) function in the eukaryotic system Saccharomyces cerevisiae . We demonstrate that the four human and soya bean ferritin chains are successfully expressed in our model system. Upon coexpression of either both human or soya bean ferritin chains, respiratory conditions along with iron supplementation led us to obtain the maximum yields of iron stored in yeast described to date. Human and soya bean ferritin chains are functional and present equivalent properties as promoters of cell survival in iron overload conditions. The best system revealed that the four human and soya bean ferritins possess a novel function as anti‐ageing proteins in conditions of iron excess. In this respect, both ferritin chains with oxidoreductase capacity (human‐H and soya bean‐H2) bear the highest capacity to extend life suggesting the possibility of an evolutionary conservation. Abstract : Clonning and overexpressing both heavy and light ferritin chains from human or soybean leads to high rates of intracellular iron chelated by these proteins, being respiratory medium the one that renders the highest performance in intracellular iron concentration Human and Soybean ferritins can be either humanised or 'vegetalised', respectively in budding yeast to demonstrate their role in life extension.