Improved Protocols for Illumina Sequencing. (2nd January 2014)

• Record Type: Journal Article
• Authors: Bronner, Iraad F.; Quail, Michael A.; Turner, Daniel J.; Swerdlow, Harold
• Editors: Haines, Jonathan L.; Korf, Bruce R.; Morton, Cynthia C.; Seidman, Christine E.; Seidman, J.G.; Smith, Douglas R.
• Is Part Of: Current protocols in human genetics. Volume 79(2013)
• Page Start: 18.2.1
• Page End: 18.2.42
• Publication Date: 2014-01-02
• Subjects: Illumina -- next‐generation -- sequencer -- protocols; Human genetics -- Laboratory manuals; Genetic Techniques; Human genetics; Laboratory manuals; 599.935028
• DOI: 10.1002/0471142905.hg1802s79
• Languages: English
• ISSNs: 1934-8258

Abstract

Abstract: In this unit, we describe a set of improvements that have been made to the standard Illumina protocols to make the sequencing process more reliable in a high‐throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data. Curr. Protoc. Hum. Genet . 79:18.2.1‐18.2.42. © 2013 by John Wiley & Sons, Inc.