Real‐time Recordings of Migrating Cortical Neurons from GFP and Cre Recombinase Expressing Mice. Issue 1 (4th January 2016)

• Record Type: Journal Article
• Authors: Tielens, Sylvia; Godin, Juliette D.; Nguyen, Laurent
• Editors: Gerfen, Charles R.; Holmes, Andrew; Sibley, David; Skolnick, Phil; Wray, Susan
• Is Part Of: Current protocols in neuroscience. Issue 74:Issue 1(2016)
• Page Start: 3.29.1
• Page End: 3.29.23
• Publication Date: 2016-01-04
• Subjects: live imaging -- migration -- neuron -- organotypic slices -- focal electroporation; Neurosciences -- Laboratory manuals; Neurosciences; Cytological Techniques; Histological Techniques; Nervous System -- anatomy & histology; Neurosciences; Laboratory Manual; Laboratory manuals; 612.8
• DOI: 10.1002/0471142301.ns0329s74
• Languages: English
• ISSNs: 1934-8576

Abstract

Abstract: The cerebral cortex is one of the most intricate regions of the brain that requires elaborate cell migration patterns for its development. Experimental observations show that projection neurons migrate radially within the cortical wall, whereas interneurons migrate along multiple tangential paths to reach the developing cortex. Tight regulation of the cell migration processes ensures proper positioning and functional integration of neurons to specific cerebral cortical circuits. Disruption of neuronal migration often leads to cortical dysfunction and/or malformation associated with neurological disorders. Unveiling the molecular control of neuron migration is thus fundamental to understanding the physiological or pathological development of the cerebral cortex. In this unit, protocols allowing detailed analysis of patterns of migration of both interneurons and projection neurons under different experimental conditions (i.e., loss or gain of function) are presented. © 2016 by John Wiley & Sons, Inc.