The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles. Issue 1 (4th January 2016)

• Record Type: Journal Article
• Authors: Revelo, Natalia H.; Rizzoli, Silvio O.
• Editors: Gerfen, Charles R.; Holmes, Andrew; Sibley, David; Skolnick, Phil; Wray, Susan
• Is Part Of: Current protocols in neuroscience. Issue 74:Issue 1(2016)
• Page Start: 2.25.1
• Page End: 2.25.21
• Publication Date: 2016-01-04
• Subjects: mCLING -- membrane trafficking -- endocytosis -- high‐resolution microscopy -- immunolabeling; Neurosciences -- Laboratory manuals; Neurosciences; Cytological Techniques; Histological Techniques; Nervous System -- anatomy & histology; Neurosciences; Laboratory Manual; Laboratory manuals; 612.8
• DOI: 10.1002/0471142301.ns0225s74
• Languages: English
• ISSNs: 1934-8576

Abstract

Abstract: mCLING is a fixable endocytosis marker that can be combined with immunolabeling techniques to study the molecular composition of trafficking organelles. mCLING can be used both in cultured cells and in tissue if critical sample preparation steps, such as fixation, are correctly performed. This unit describes protocols for the application of mCLING and for the subsequent sample processing. We include immunostaining protocols and embedding procedures for confocal and high‐resolution microscopy. © 2016 by John Wiley & Sons, Inc.