Detection and Quantification of Neurotransmitters in Dialysates. Issue 1 (1st April 2013)

• Record Type: Journal Article
• Authors: Zapata, Agustin; Chefer, Vladimir I.; Parrot, Sandrine; Denoroy, Luc
• Editors: Crawley, Jacqueline N.; Gerfen, Charles R.; Rogawski, Michael A.; Sibley, David R.; Skolnick, Phil; Wray, Susan
• Is Part Of: Current protocols in neuroscience. Issue 63:Issue 1(2013)
• Page Start: 7.4.1
• Page End: 7.4.35
• Publication Date: 2013-04-01
• Subjects: high‐performance liquid chromatography (HPLC) -- electrochemical detection -- capillary electrophoresis -- laser fluorescence; Neurosciences -- Laboratory manuals; Neurosciences; Cytological Techniques; Histological Techniques; Nervous System -- anatomy & histology; Neurosciences; Laboratory Manual; Laboratory manuals; 612.8
• DOI: 10.1002/0471142301.ns0704s63
• Languages: English
• ISSNs: 1934-8576

Abstract

Abstract: Sensitive analytical methods are needed for the separation and quantification of neurotransmitters obtained in microdialysate studies. This unit describes methods that permit quantification of nanomolar concentrations of monoamines and their metabolites (high‐performance liquid chromatography [HPLC] electrochemical detection), acetylcholine (HPLC‐coupled to an enzyme reactor), and amino acids (HPLC‐fluorescence detection, capillary electrophoresis with laser‐induced fluorescence detection). Curr. Protoc. Neurosci . 63:7.4.1‐7.4.35. © 2013 by John Wiley & Sons, Inc.