Fast and Efficient Neural Conversion of Human Hematopoietic Cells. (8th November 2016)

• Record Type: Journal Article
• Authors: Bruzos‐Cidon, Cristina; Castaño, Julio; Torrecilla, Maria; Sanchez‐Pernaute, Rosario; Giorgetti, Alessandra
• Editors: Bhatia, Mick; Elefanty, Andrew G.; Fisher, Susan J.; Patient, Roger; Schlaeger, Thorsten; Snyder, Evan Y.
• Is Part Of: Current protocols in stem cell biology. Volume 39(2016)
• Page Start: 1F.15.1
• Page End: 1F.15.20
• Publication Date: 2016-11-08
• Subjects: cord blood -- direct conversion -- hematopoietic cells -- induced neurons -- reprogramming -- Sendai virus; Stem cells -- Laboratory manuals; Stem cells -- Physiology; Stem Cells; Stem cells; Laboratory Manual; Laboratory manuals; 571.5
• DOI: 10.1002/cpsc.16
• Languages: English
• ISSNs: 1941-7322

Abstract

Abstract: A major challenge in regenerative medicine is the generation of functionally effective target cells to replace or repair damaged tissues. The finding that most somatic cells can be directly converted into cells of another lineage by the expression of specific transcription factors has paved the way to novel applications. Induced neurons (iNs) represent an alternative source of neurons for disease modeling, drug screening, and potentially, for cell replacement therapy. This unit describes methods for the efficient conversion of blood cells into iNs, including protocols to isolate cord blood CD133+ cells, infect them with Sendai virus vectors that express SOX2 and c‐MYC, and differentiate the infected cells (PB‐MNCs) into mature neurons. A method to reprogram peripheral blood mononuclear cells into iNs is also described. Support protocols describe how to culture rat astrocytes and characterize the electrophysiology of iNs. © 2016 by John Wiley & Sons, Inc.