Antithrombotic Therapy by Regulating the ROS‐Mediated Thrombosis Microenvironment and Specific Nonpharmaceutical Thrombolysis Using Prussian Blue Nanodroplets. Issue 15 (4th March 2022)

• Record Type: Journal Article
• Authors: Zhang, Wenli; Wang, Junrui; Xie, Zhuoyan; Zou, Hongmi; Chen, Qiaoqi; Xu, Lian; Hu, Liu; Fang, Ni; Xu, Jie; Zhou, Jun; Liu, Jia; Ran, Haitao; Wang, Zhigang; Zhang, Yu; Guo, Dajing
• Is Part Of: Small. Volume 18:Issue 15(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-03-04
• Subjects: thrombosis -- Prussian blue -- microenvironment regulation -- optical droplet vaporization -- photothermal therapy; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202106252
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: In thrombotic diseases, the effects of reactive oxygen species (ROS)‐mediated oxidative stress as a "perpetrator" in thrombosis must be resolved. Accordingly, an insufficient understanding of thrombus therapy prompted the authors to pursue a more comprehensive and efficient antithrombotic treatment strategy. A Prussian blue (PB)‐based nanodroplet system (PB‐PFP@PC) is designed using PB and perfluorinated pentane (PFP) in the core, and a targeting peptide (CREKA, Cys‐Arg‐Glu‐Lys‐Ala) is attached to poly(lactic‐coglycolic acid) (PLGA) as the delivery carrier shell. Upon near‐infrared (NIR) laser irradiation, PB and PFP jointly achieve an unprecedented dual strategy for drug‐free thrombolysis: photothermal therapy (PTT) combined with optical droplet vaporization (ODV). PB, a nanoenzyme, also regulates the vascular microenvironment via its antioxidant activity to continuously scavenge abnormally elevated ROS and correspondingly reduce inflammatory factors in the thrombus site. This study provides a demonstration of not only the potential of ODV in thrombus therapy but also the mechanism underlying PTT thrombolysis due to thermal ablation‐induced fibrin network structural damage. Moreover, PB catalyzes ROS to generate oxygen (O2 ), which combines with the ODV effect, enhancing the ultrasound signal. Thus, regulation of the thrombosis microenvironment combined with specific nonpharmaceutical thrombolysis by PB nanodroplets provides a more comprehensive and efficient antithrombotic therapeutic strategy. Abstract : PB‐based nanodroplets (PB‐PFP@PC) actively target the thrombus site; upon near‐infrared (NIR) laser irradiation, PB and PFP jointly achieve an unprecedented dual strategy for drug‐free thrombolysis: photothermal therapy (PTT) combined with optical droplet vaporization (ODV). Simultaneously, PB‐PFP@PC nanodroplets regulate the thrombosis microenvironment, reduce the levels of corresponding inflammatory factors, and provide a comprehensive and efficient antithrombotic therapeutic strategy.