Nanocarriers Made of Proteins: Intracellular Visualization of a Smart Biodegradable Drug Delivery System. Issue 15 (27th February 2022)

• Record Type: Journal Article
• Authors: Frey, Marie‐Luise; Han, Shen; Halim, Henry; Kaltbeitzel, Anke; Riedinger, Andreas; Landfester, Katharina; Lieberwirth, Ingo
• Is Part Of: Small. Volume 18:Issue 15(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-27
• Subjects: correlative microscopy -- drug delivery -- intracellular pathways -- protein nanocarriers -- transmission electron microscopy; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202106094
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: This work analyzes the intracellular fate of protein‐based nanocarriers along their endolysosomal pathway by means of correlative light and electron microscopy methods. To unambiguously identify the nanocarriers and their degradation remnants in the cellular environment, they are labeled with fluorescent, inorganic nanoplatelets. This allows tracking the nanocarriers on their intracellular pathway by means of electron microscopy imaging. From the present data, it is possible to identify different cell compartments in which the nanocarriers are processed. Finally, three different terminal routes for the intracellular destiny of the nanocarriers are presented. These findings are important to reveal the degradation process of protein nanocapsules and contribute to the understanding of the therapeutic success of an encapsulated drug. Abstract : Nanocapsules made of a protein and additionally labeled with CdSe/CdZnS nanoplatelets are examined as a model system for drug delivery. By correlating the fluorescent label with the high‐resolution electron microscopy image, the intracellular pathway of the protein nanocapsules is revealed showing the degradation of the capsule material.