In Situ Hybridization to Identify Gut Stem Cells. (3rd August 2015)

• Record Type: Journal Article
• Authors: Gregorieff, Alex; Clevers, Hans
• Editors: Bhatia, Mick; Elefanty, Andrew G.; Fisher, Susan J.; Patient, Roger; Schlaeger, Thorsten; Snyder, Evan Y.
• Is Part Of: Current protocols in stem cell biology. Volume 34(2015)
• Page Start: 2F.1.1
• Page End: 2F.1.11
• Publication Date: 2015-08-03
• Subjects: (ISH) in situ hybridization -- digoxigenin RNA probes -- formalin‐fixed paraffin‐embedded -- intestinal sections; Stem cells -- Laboratory manuals; Stem cells -- Physiology; Stem Cells; Stem cells; Laboratory Manual; Laboratory manuals; 571.5
• DOI: 10.1002/9780470151808.sc02f01s34
• Languages: English
• ISSNs: 1941-7322

Abstract

Abstract: In recent years, considerable effort has been directed toward identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution. © 2015 by John Wiley & Sons, Inc.