Detection of Neurofilament Light Chain with Label‐Free Electrolyte‐Gated Organic Field‐Effect Transistors. Issue 11 (10th February 2022)

• Record Type: Journal Article
• Authors: Solodka, Kateryna; Berto, Marcello; Ferraro, Diana; Menozzi, Claudia; Borsari, Marco; Bortolotti, Carlo A.; Biscarini, Fabio; Pinti, Marcello
• Is Part Of: Advanced materials interfaces. Volume 9:Issue 11(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-10
• Subjects: electrolyte‐gated organic field‐effect transistors -- Guggenheim–Anderson–De Boer isotherm -- multiple sclerosis -- neurofilament light chain; Materials science -- Periodicals; 620.11
• DOI: 10.1002/admi.202102341
• Languages: English
• ISSNs: 2196-7350

Abstract

Abstract: Neurofilaments are structural scaffolding proteins of the neuronal cytoskeleton. Upon axonal injury, the neurofilament light chain (NF‐L) is released into the interstitial fluid and eventually reaches the cerebrospinal fluid and blood. Therefore, NF‐L is emerging as a biomarker of neurological disorders, including neurodegenerative dementia, Parkinson's disease, and multiple sclerosis. It is challenging to quantify NF‐L in bodily fluids due to its low levels. This work reports the detection of NF‐L in aqueous solutions with an organic electronic device. The biosensor is based on the electrolyte‐gated organic field‐effect transistor (EGOFET) architecture and can quantify NF‐L down to sub‐pM levels; thanks to modification of the device gate with anti‐NF‐L antibodies imparted with potentially controlled orientation. The response is fitted to the Guggenheim–Anderson–De Boer adsorption model to describe NF‐L adsorption at the gate/electrolyte interface, to consider the formation of a strongly adsorbed protein layer bound to the antibody and the formation of weakly bound NF‐L multilayers, an interpretation which is also backed up by morphological characterization via atomic force microscopy. The label‐free, selective, and rapid response makes this EGOFET biosensor a promising tool for the diagnosis and monitoring of neuronal damages through the detection of NF‐L in physio‐pathological ranges. Abstract : The detection of neurofilaments light chain (NF‐L) in aqueous solutions with an electrolyte‐gated organic field‐effect transistor sensor down to sub‐pm levels is reported. The response is fitted to the Guggenheim–Anderson–De Boer adsorption model to describe NF‐L adsorption at the gate/electrolyte interface and the formation of NF‐L multilayers.