Β‐Galactosidase: From its source and applications to its recombinant form. (16th March 2021)

• Record Type: Journal Article
• Authors: Movahedpour, Ahmad; Ahmadi, Nahid; Ghalamfarsa, Farideh; Ghesmati, Zeinab; Khalifeh, Masoomeh; Maleksabet, Amir; Shabaninejad, Zahra; Taheri‐Anganeh, Mortaza; Savardashtaki, Amir
• Is Part Of: Biotechnology and applied biochemistry. Volume 69:Number 2(2022)
• Page Start: 612
• Page End: 628
• Publication Date: 2021-03-16
• Subjects: enzyme -- lactose -- recombinant protein; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.2137
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: Carbohydrate‐active enzymes are a group of important enzymes playing a critical role in the degradation and synthesis of carbohydrates. Glycosidases can hydrolyze glycosides into oligosaccharides, polysaccharides, and glycoconjugates via a cost‐effective approach. Lactase is an important member of β‐glycosidases found in higher plants, animals, and microorganisms. β‐Galactosidases can be used to degrade the milk lactose for making lactose‐free milk, which is sweeter than regular milk and is suitable for lactose‐intolerant people. β‐Galactosidase is employed by many food industries to degrade lactose and improve the digestibility, sweetness, solubility, and flavor of dairy products. β‐Galactosidase enzymes have various families and are applied in the food‐processing industries such as hydrolyzed‐milk products, whey, and galactooligosaccharides. Thus, this enzyme is a valuable protein which is now produced by recombinant technology. In this review, origins, structure, recombinant production, and critical modifications of β‐galactosidase for improving the production process are discussed. Since β‐galactosidase is a valuable enzyme in industry and health care, a study of its various aspects is important in industrial biotechnology and applied biochemistry. Abstract : β‐galactosidase converts lactose to glucose and galactose.