Enhancement and mechanisms of MC3T3‐E1 osteoblast‐like cell adhesion to albumin through calcium exposure. (17th March 2021)

• Record Type: Journal Article
• Authors: Haag, Stephanie L.; Schiele, Nathan R.; Bernards, Matthew T.
• Is Part Of: Biotechnology and applied biochemistry. Volume 69:Number 2(2022)
• Page Start: 492
• Page End: 502
• Publication Date: 2021-03-17
• Subjects: albumin -- calcium -- integrin binding -- MC3T3‐E1 adhesion -- tissue engineering; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.2126
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: Serum albumin is the most prominent protein in blood, and it aids in bone fracture healing, though the manner through which enhanced healing occurs is not well understood. This study investigates the influence of calcium on the bioactivity of albumin due to the prevalence of calcium at bone injury sites. Bovine serum albumin (BSA) was exposed to varying concentrations of calcium, adsorbed to tissue culture polystyrene, and the subsequent BSA‐coated surfaces were evaluated with calcium titration, and cell adhesion, viability, and binding inhibition studies. Calcium‐modified BSA improved overall MC3T3‐E1 osteoblast‐like cell adhesion, although high calcium concentrations induced cell death. Inhibiting specific integrins revealed that without calcium exposure, cell binding to BSA was primarily mediated by integrins that typically bind to the GFOGER sequence of collagen. As calcium exposure increases, the primary binding interaction transitioned to integrins known to bind RGD. However, cell binding to calcium‐modified BSA was not completely eliminated during the inhibition studies indicating additional unidentified binding interactions occur. Overall, these results suggest that the exposure to calcium induces conformational changes that affect the cell‐binding bioactivity of BSA, which may explain the beneficial impact of albumin in bone tissue. Abstract : Bovine serum albumin exposed to Increasing levels of calcium facilitates greater MC3T3‐E1 adhesion, albeit through changing integrin mediated interactions.