Circulating tumor DNA in primary mediastinal large B-cell lymphoma versus classical Hodgkin lymphoma: a retrospective study. Issue 4 (21st March 2022)

• Record Type: Journal Article
• Authors: Camus, Vincent; Viennot, Mathieu; Lévêque, Emilie; Viailly, Pierre-Julien; Tonnelet, David; Veresezan, Elena-Liana; Drieux, Fanny; Etancelin, Pascaline; Dubois, Sydney; Stamatoullas, Aspasia; Tilly, Hervé; Bohers, Elodie; Jardin, Fabrice
• Is Part Of: Leukemia & lymphoma. Volume 63:Issue 4(2022)
• Page Start: 834
• Page End: 844
• Publication Date: 2022-03-21
• Subjects: Primary mediastinal large B cell lymphoma -- classical Hodgkin lymphoma -- circulating tumor DNA -- cell-free DNA; Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.1080/10428194.2021.2010060
• Languages: English
• ISSNs: 1042-8194

Abstract

Abstract: Few data exist concerning circulating tumor DNA (ctDNA) relevance in primary mediastinal B-cell lymphoma (PMBL). To explore this topic, we applied a 9-gene next-generation sequencing pipeline to samples from forty-four PMBL patients (median age 36.5 years). The primary endpoint was a similarity between paired biopsy/plasma mutational profiles. We detected at least one variant in 32 plasma samples (80%). The similarity between the biopsy and ctDNA genetic profiles for the 30 patients with paired mutated biopsy/plasma samples was greater than or equal to 80% in 19 patients (63.3%). We then compared PMBL ctDNA features with those of a cohort of Hodgkin lymphoma patients ( n = 60). The top three mutated genes were SOCS1, TNFAIP3, and B2M in both lymphoma types. PMBL displayed more alterations in TNFAIP3 (71.9% vs. 46.3%, p = 0.029) and GNA13 (46.9% vs. 17.1%, p = 0.013) than cHL. Our 9-gene set may delineate tumor genotypes using ctDNA samples from both lymphoma types.