A Quick Viability Test Protocol for Hemp (Cannabis Sativa L.) Seeds. Issue 4 (3rd April 2022)

• Record Type: Journal Article
• Authors: Rachel Jacob, Sherry; Mishra, Aradhana; Kumari, Manju; Bhatt, K.C.; Gupta, Veena; Singh, Kuldeep
• Is Part Of: Journal of natural fibers. Volume 19:Issue 4(2022)
• Page Start: 1281
• Page End: 1286
• Publication Date: 2022-04-03
• Subjects: Hemp -- seed viability -- tetrazolium staining; 麻 -- 种子活力 -- 四唑染色法 -- 大麻种子短标题-生活力筛选; Textile research -- Periodicals; Textile research; Periodicals; 677.005
• DOI: 10.1080/15440478.2020.1764451
• Languages: English
• ISSNs: 1544-0478

Abstract

ABSTRACT: The viability testing in hemp seeds is a crucial step in its seed supply chain. Based on the legal status of hemp cultivation, each provincial area would benefit from the viability assessment, either by screening out dead seeds in legalized zones or by detecting the viable seeds in areas where hemp cultivation is not legalized. The routine procedure of assessing seed viability through germination tests takes a minimum of 7 days, which is practically not beneficial in the seed supply system. There is a need for developing a quick, reproducible, and fool-proof technique whereby seed lots can be rapidly screened, utilizing minimum resources. In this experiment, we have standardized a tetrazolium-based viability testing protocol which can identify the viability status of seed lots within 3 h.