Biocatalytic assembly of chemically modified oligonucleotides. (16th March 2022)

• Record Type: Journal Article
• Authors: Mann, Greg; Stanger, Frédéric V.; Peschke, Theo; Schroer, Kirsten; Trung, Nhat Quang Nguyen; Burrer, Nathalie; Grassinger, Franziska; Piccioni, Lorenzo; Erdogan, Oemer; Heimburger, Jeremy; Koehl, Hervé; Ertle, Heike; Denay, Regis; Dreier, Philippe; Schlama, Thierry
• Is Part Of: Tetrahedron letters. Volume 93(2022)
• Publication Date: 2022-03-16
• Subjects: Oligonucleotides -- Biocatalysis -- Sustainable -- Ligation; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tetlet.2022.153696
• Languages: English
• ISSNs: 0040-4039

Abstract

Graphical abstract: Abstract: With renewed interest in therapeutic oligonucleotides, and at the same time the pharmaceutical industry set itself ambitious sustainability goals, there is a strong interest to investigate next-generation synthesis methods for these highly modified oligonucleotides. We herein report an RNA ligase catalyzed assembly of multiple, short oligonucleotides in aqueous solution to generate an siRNA product. A conversion of the non-native substrates to the desired product of 40 – 80 % was achieved.