A comparison study of physiological response and TaZIPs expression in seedlings of two wheat (Triticum aestivum L.) cultivars with contrasting grain zinc accumulation. (May 2022)

• Record Type: Journal Article
• Authors: Li, Guangxin; Wang, Yun; Liu, Hongen; Qin, Shiyu; Sui, Fuqing; Fu, Haichao; Duan, Ran; Li, Chang; Zhao, Peng
• Is Part Of: Plant science. Volume 318(2022)
• Publication Date: 2022-05
• Subjects: Wheat (Triticum aestivum L.) -- Zinc uptake -- Subcellular fractionation -- TaZIPs transporter proteins -- Gene expression; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2022.111237
• Languages: English
• ISSNs: 0168-9452

Abstract

Abstract: Screening and breeding of high-Zn-accumulating wheat cultivars have received increasing attention in recent years. However, the exact mechanism of Zn uptake and accumulation in wheat is not fully understood. Here, we investigated the physiological responses and TaZIPs gene expression in a low (Zhengmai0856, ZM0856) and a high (Aikang58, AK58) grain-Zn-accumulating wheat cultivars under hydroponic conditions with different levels of Zn supply. Results showed that AK58 was a Zn sensitive cultivar with better growth advantage, while ZM0856 was a Zn tolerant cultivar with higher capacity of Zn uptake. In addition, gene expression analysis showed that, the expression levels of the TaZIP3, TaZIP5, and TaZIP7 in roots were increased in both cultivars under Zn deficiency. In shoots, TaZIP3 and TaZIP 6 transcript accumulation was lower in AK58 than ZM0856, whereas TaZIP7 showed the opposite effect. Moreover, multivariate statistical analysis (Pearson's correlation and PCA) showed that the mechanisms involved in Zn uptake and translocation was closely related to subcellular biosynthesis and ZIP gene expression regulation, whereas adequate Zn supply improved the Zn uptake and root-to-shoot translocation. These novel findings might be helpful for the molecular-assisted selecting and breeding of Zn-rich wheat cultivars. Highlights: AK58 showed better performance than ZM0856 under different Zinc concentrations. Zinc supply reduced the uptake of Fe, Mn, and Cu by both wheat cultivars in the root. Zinc supply alleviated Zn-deficiency-induced photosynthetic damage and oxidative stress for both wheat cultivars. Subcellular (cell wall, cell soluble fraction) plays an important role in sequestration and tolerance of high Zn ions. Zinc uptake and translocation were mediated by the induced expression of these TaZIPs transporters in wheat.