Algiphilus acroporae sp. nov. and Coraliihabitans acroporae gen. nov. sp. nov., isolated from scleractinian coral Acropora digitifera. Issue 4 (19th April 2022)

• Record Type: Journal Article
• Authors: Sun, Hao; Zheng, Huina; Liao, Baolin; Chen, Bogui; Li, Aihua; Xiao, Baohua
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 4(2022)
• Publication Date: 2022-04-19
• Subjects: Algiphilus acroporae sp. nov. Coraliihabitans acroporae gen. nov. sp. nov -- Acropora digitifera -- scleractinian coral; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005321
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Two Gram-staining-negative, aerobic, rod-shaped bacteria NNCM1 T and NNCM2 T were isolated from the scleractinian coral Acropora digitifera . NNCM1 T grew with 0.5–12 % (w/v) NaCl (optimum, 3–6 %), at 18–37 °C (optimum, 28 °C) and at pH 6.0–10.0 (optimum, 7.0–8.0). NNCM2 T grew with 0.5–10 % (w/v) NaCl (optimum, 2 %), at 18–37 °C (optimum, 28 °C) and at pH 6.5–9.0 (optimum, 7.0). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that NNCM1 T formed a lineage within the genus Algiphilus of the family Algiphilaceae, and it was distinct from the most closely related species Algiphilus aromaticivorans DG1253 T, with a 16S rRNA gene sequences similarity of 97.05 %. NNCM2 T formed a lineage within the family Rhodobacteraceae, and it was distinct from the closely related genera Limibaculum halophilum CAU 1123 T, Paroceanicella profunda D4M1 T and Pseudoruegeria aestuarii MME-001 T with 93.41, 92.78 and 91.09% identities, respectively. The major respiratory quinone was Q-8 and Q-10 for NNCM1 T and NNCM2 T, respectively. The predominant fatty acids (more than 10 %) were summed feature 8 (39.4 %) and C16 : 0 (19.4 %) for NNCM1 T and summed feature 8 (62.8 %) and C16 : 0 (12.4 %) for NNCM2 T . The DNA G+C contents of NNCM1 T and NNCM2 T were 63.3 and 63.4 mol% respectively. The polar lipids of NNCM1 T comprised one diphosphatidylglycerol, one phosphatidylethanolamine, one phosphatidylglycerol and one unknown polar lipid, while those of NNCM2 T comprised one phosphatidylethanolamine, one phosphatidylglycerol, one aminolipid and four unknown polar lipids. Phenotypic characteristics (physiological, biochemical and chemotaxonomic) also supported the taxonomic novelty of the two isolates. Thus, NNCM1 T is considered to represent a novel species within genus Algiphilus, for which the name Algiphilus acroporae sp. nov. is proposed. The type strain is NNCM1 T (=KCTC 82966 T =MCCC 1K06445 T ). NNCM2 T represents a novel genus and species within the family Rhodobacteraceae, for which the name Coraliihabitans acroporae gen. nov. sp. nov. is proposed. The type strain is NNCM2 T (=KCTC 82967 T =MCCC 1K06408 T ).