Coactive role of zinc oxide nanoparticles and plant growth promoting rhizobacteria for mitigation of synchronized effects of heat and drought stress in wheat plants. (June 2022)

• Record Type: Journal Article
• Authors: Azmat, Ammar; Tanveer, Yashfa; Yasmin, Humaira; Hassan, Muhammad Nadeem; Shahzad, Asim; Reddy, Munagala; Ahmad, Ajaz
• Is Part Of: Chemosphere. Volume 297(2022)
• Publication Date: 2022-06
• Subjects: Zinc oxide nanoparticles -- Drought stress -- Heat stress -- Wheat -- Papaya fruit extract -- Antioxidant defense; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2022.133982
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: This study intended to investigate the potential of the plant growth-promoting rhizobacteria (PGPR) and green synthesized zinc oxide nanoparticles (ZnO-NPs) (fruit extract of Papaya) against heat and drought stress in wheat. The characterization of green-synthesized ZnO-NPs was done through UV–vis spectrophotometry, Fourier-transform infrared spectrometry, X-ray diffraction and scanning electron microscopy. Individual and combination of PGPR ( Pseudomonas sp . ) and ZnO-NPs (10 ppm) amendments were tested in a pot experiment to upregulate wheat defence system under three stress groups (drought, heat and combined heat and drought stress). Drought and heat stress synergistically caused higher damage to wheat plants than individual heat and drought stress. This observation was confirmed with remarkable higher MDA and hydrogen peroxide (H2 O2 ) content. Treated plants exposed to all stress groups showed an improved wheat growth and stress resistance through better biomass, photosynthetic pigments, nutrients, soluble sugars, protein and indole acetic acid content. Combination of ZnO-NPs and Pseudomonas sp. Protects the plants from all stress groups by producing higher proline, antioxidant enzymes i. e superoxide dismutase, peroxidase, catalase, ascorbate peroxidase, glutathione reductase and dehydroascorbate reductase, and abscisic acid. Moreover, higher stress alleviation by this treatment was manifested by marked reduced electrolyte leakage, MDA and H2 O2 . The findings of current study confirmed that the synergistic actions of PGPR and ZnO-NPs can rescue plants from both single and combined heat and drought stress. Graphical abstract: Image 1 Highlights: Wheat plants are adversely affected by drought stress than heat stress. Plants exposed to combined stress exhibited more harmful effects. ZnO-NPs reduce drought and heat stress by improving biochemical metabolism. Rhizobacteria impart stress resistance by enhanced osmoregulation and antioxidants. Co-application of Zn-NPs and PGPR induce more tolerance in wheat.