Effect of daily co-exposure to inulin and chlorpyrifos on selected microbiota endpoints in the SHIME® model. (1st June 2022)

• Record Type: Journal Article
• Authors: Joly Condette, Claire; Djekkoun, Narimane; Reygner, Julie; Depeint, Flore; Delanaud, Stephane; Rhazi, Larbi; Bach, Veronique; Khorsi-Cauet, Hafida
• Is Part Of: Environmental pollution. Volume 302(2022)
• Publication Date: 2022-06-01
• Subjects: Prebiotic -- Chlorpyrifos -- In vitro intestine -- Microbiota perturbations -- Microbial metabolites -- TTGE; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.118961
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: The intestinal microbiota has a key role in human health via the interaction with the somatic and immune cells in the digestive tract environment. Food, through matrix effect, nutrient and non-nutrient molecules, is a key regulator of microbiota diversity. As a food contaminant, the pesticide chlorpyrifos (CPF) has an effect on the composition of the intestinal microbiota and induces perturbation of microbiota. Prebiotics (and notably inulin) are known for their ability to promote an equilibrium of the microbiota that favours saccharolytic bacteria. The SHIME® dynamic in vitro model of the human intestine was exposed to CPF and inulin concomitantly for 30 days, in order to assess variations in both the bacterial populations and their metabolites. Various analyses of the microbiota (notably temporal temperature gradient gel electrophoresis) revealed a protective effect of the prebiotic through inhibition of the enterobacterial ( E. coli) population. Bifidobacteria were only temporarily inhibited at D15 and recovered at D30. Although other potentially beneficial populations (lactobacilli) were not greatly modified, their activity and that of the saccharolytic bacteria in general were highlighted by an increase in levels of short-chain fatty acids and more specifically butyrate. Given the known role of host-microbiota communication, CPF's impact on the body's homeostasis remains to be determined. Graphical abstract: Image 1 Highlights: SHIME® in vitro model of the human intestine was exposed to chlorpyrifos (CPF) and inulin. CPF alters the microbiota ecosystem, inulin partially prevents the effects on the microbiota and its metabolism. Nutrition intervention may counteract some harmful impact of pesticides.