Computational Modeling and Experimental Characterization of Extrusion Printing into Suspension Baths. Issue 7 (20th November 2021)

• Record Type: Journal Article
• Authors: Prendergast, Margaret E.; Burdick, Jason A.
• Other Names: Anseth Kristi S. guestEditor.; Xia Younan guestEditor.
• Is Part Of: Advanced healthcare materials. Volume 11:Issue 7(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-11-20
• Subjects: 3D bioprinting -- biofabrication -- extrusion printing -- suspension baths; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.202101679
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: The extrusion printing of inks into suspension baths is an exciting tool, as it allows the printing of diverse and soft hydrogel inks into 3D space without the need for layer‐by‐layer fabrication. However, this printing process is complex and there have been limited studies to experimentally and computationally characterize the process. In this work, hydrogel inks (i.e., gelatin methacrylamide (GelMA)), suspension baths (i.e., agarose, Carbopol), and the printing process are examined via rheological, computational, and experimental analyses. Rheological data on various hydrogel inks and suspension baths is utilized to develop computational printing simulations based on Carreau constitutive viscosity models of the printing of inks within suspension baths. These results are then compared to experimental outcomes using custom print designs where features such as needle translation speed, defined in this work as print speed, are varied and printed filament resolution is quantified. Results are then used to identify print parameters for the printing of a GelMA ink into a unique guest–host hyaluronic acid suspension bath. This work emphasizes the importance of key rheological properties and print parameters for suspension bath printing and provides a computational model and experimental tools that can be used to inform the selection of print settings. Abstract : Extrusion printing of inks into suspension baths, which allows for the printing of various soft hydrogel inks into 3D space, is a promising technology for the biofabrication field. In this work, a series of popular suspension baths and inks are characterized through experimental, rheological, and computational analyses to elucidate key rheological properties and print parameters for suspension bath printing.