A phylogenetically unresolved apicomplexan (APXSc) causing swirl lesions in the Tehuelche scallop, Aequipecten tehuelchus, from the Southwest Atlantic coast. (April 2022)

• Record Type: Journal Article
• Authors: Vázquez, Nuria; Freeman, Mark A.; Cremonte, Florencia; Gilardoni, Carmen; Kristmundsson, Árni
• Is Part Of: International journal for parasitology. Volume 17(2022)
• Page Start: 295
• Page End: 307
• Publication Date: 2022-04
• Subjects: Apicomplexan scallop -- Aequipecten tehuelchus -- Histopathology -- Phylogeny -- Ultrastructure; Parasites -- Periodicals; Parasitology -- Periodicals; Animals -- Periodicals; Wildlife diseases -- Periodicals; Parasites -- Periodicals; Animals, Wild -- Periodicals; Animals; Parasites; Parasitology; Wildlife diseases; Periodicals; 591.7857
• DOI: 10.1016/j.ijppaw.2022.03.009
• Languages: English
• ISSNs: 2213-2244

Abstract

Abstract: The study reports a previously unknown apicomplexan (APXSc) parasite infecting wild scallops Aequipecten tehuelchus (d'Orbigny, 1842) from two separate areas (La Tapera and Punta Conos) of the San José gulf, in Patagonia Argentina. Histology, transmission electron microscope, molecular analyses and in situ hybridization were performed to describe the morphology of APXSc, and confirm its phylogenetic status. The prevalence of APXSc infection was 24% and 72% in scallops from La Tapera and Punta Conos, respectively. Seasonal variation was observed for scallops from La Tapera, recording highest prevalence in summer. A positive relationship between the presence of the APXSc and the size of the scallops was observed. A SSU rDNA consensus sequence of 1758 base pairs was generated which has a 94.8% identity to sequences obtained from a pathogenic apicomplexan parasite infecting Ostrea chilensis in New Zealand, but not closely related to other apicomplexans. The asexual reproduction, i.e. merogony, occurs in the Tehuelche scallop whilst the gamogonic and sporogonic stages were absent, suggesting a yet unknown definitive host. Severe host inflammation response involving fibroblast-like hemocytes surrounding the APXSc in the form of granuloma-like "swirls" is characteristic for this apicomplexan infection. Further studies are needed to reveal the life cycle, and presumable pathogenicity of APXSc. Graphical abstract: Image 1 Highlights: A novel apicomplexan (APXSc) parasite infecting wild scallops Aequipecten tehuelchus . Positive relationship between the presence of the APXSc with the colder season and the size of the scallops. SSU rDNA sequence matched with 94.8% identity to sequences from an apicomplexan infecting Ostrea chilensis in New Zealand. The Tehuelche scallop is the intermediate host for APXSc.