Interaction between laccase and diethylstilbestrol based on multispectral and chromatography analyses. Issue 5 (31st January 2022)

• Record Type: Journal Article
• Authors: Lin, Xiaolian; Liu, Hongyan; Tang, Lin; Shi, Mengjie; Xu, Minhua; Huang, Yipeng; Yi, Zhongsheng; Chen, Huiying
• Is Part Of: Journal of molecular recognition. Volume 35:Issue 5(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-01-31
• Subjects: diethylstilbestrol -- interaction -- laccase -- multispectroscopic methods; Molecular recognition -- Periodicals; Models, Molecular -- Periodicals; Molecular Conformation -- Periodicals; Molecular Sequence Data -- Periodicals; Molecular Structure -- Periodicals; Carrier Proteins -- Periodicals; 572.8
• DOI: 10.1002/jmr.2951
• Languages: English
• ISSNs: 0952-3499

Abstract

Abstract: Diethylstilbestrol (DES) is a synthetic form of oestrogen that does not easily degrade in the environment and can be harmful to human health. Herein, the mechanism of the interaction between laccase and DES was investigated by various spectroscopic means and high‐performance liquid chromatography (HPLC). The results of fluorescence experiments showed that the quenching of intrinsic fluorescence of laccase by DES was due to a static quenching, forming a binding site. According to the Förster non‐radiative energy transfer theory (FRET), the action distance R 0 between DES and laccase was 4.708 nm, r was 5.81 nm, and the energy transfer efficiency E was 22.08%, respectively. Both UV‐Vis absorption spectra and FT‐IR spectra indicated changes in the conformation and surroundings of the enzyme and changed in the secondary structure of laccase. Multispectral synthesis showed that the interaction of laccase with DES caused a change in the secondary structure of laccase. The degradation experiments showed that laccase could degrade DES, and the DES content decreased with time. This study provides a new theoretical basis and experimental method for further research on the reaction mechanism of the laccase degradation of DES. It may also provide a reference basis for human biological and environmental safety evaluations. Abstract : Interaction between laccase and diethylstilbestrol based on multispectral and chromatography analyses.