Long noncoding RNA EIF1AX‐AS1 promotes endometrial cancer cell apoptosis by affecting EIF1AX mRNA stabilization. Issue 4 (7th February 2022)

• Record Type: Journal Article
• Authors: Lv, Chengyu; Sun, Jiandong; Ye, Yuhong; Lin, Zihang; Li, Hua; Liu, Yue; Mo, Kaien; Xu, Weiwei; Hu, Weitao; Draz, Eman; Wang, Shie
• Is Part Of: Cancer science. Volume 113:Issue 4(2022)
• Page Start: 1277
• Page End: 1291
• Publication Date: 2022-02-07
• Subjects: apoptosis -- EIF1AX -- endometrial cancer -- long noncoding RNA -- RNA binding protein; Cancer -- Periodicals; Neoplasms -- Periodicals; Research -- Periodicals; Electronic journals; 616.994005
• DOI: 10.1111/cas.15275
• Languages: English
• ISSNs: 1347-9032

Abstract

Abstract: Long noncoding RNAs (lncRNAs) have been found to play an important role in the occurrence and development of endometrial carcinoma (EC). Here, using RNA sequencing analysis, we systemically screened and identified the lncRNA eukaryotic translation initiation factor 1A, X‐linked (EIF1AX)‐AS1, which is aberrantly downregulated in clinical EC tissues and closely correlated with tumor type. EIF1AX‐AS1 markedly inhibited EC cell proliferation and promoted apoptosis in vitro and in vivo . Mechanistically, EIF1AX‐AS1 interacts with EIF1AX mRNA and poly C binding protein 1 (PCBP1), which promote EIF1AX mRNA degradation. Intriguingly, by interacting with internal ribosome entry site‐related protein Y‐box binding protein 1 (YBX‐1), EIF1AX promotes c‐Myc translation through the internal ribosome entry site pathway. c‐Myc promotes EIF1AX transcription and thus forms a feed‐forward loop to regulate EC cell proliferation. Taken together, these data reveal new insights into the biology driving EC proliferation and highlights the potential of lncRNAs as biomarkers for prognosis and future therapeutic targets for cancer. Abstract : Long noncoding RNA EIF1AX‐AS1 is aberrantly downregulated in clinical endometrial carcinoma tissues. We describe in this paper the effect of EIF1AX‐AS1 on apoptosis in endometrial cancer cells, the mechanisms of which could relate to the promotion of EIF1AX mRNA degradation by interacting with PCBP1, and thus regulate c‐Myc mediated cell apoptosis through the internal ribosome entry site pathway.