Bioaugmentation with well-constructed consortia can effectively alleviate ammonia inhibition of practical manure anaerobic digestion. (15th May 2022)

• Record Type: Journal Article
• Authors: Yang, Ziyi; Sun, Hangyu; Zhou, Ling; Arhin, Samuel Gyebi; Papadakis, Vagelis G.; Goula, Maria A.; Liu, Guangqing; Zhang, Yi; Wang, Wen
• Is Part Of: Water research. Volume 215(2022)
• Publication Date: 2022-05-15
• Subjects: Bioaugmentation -- Acclimatized consortia -- Well-constructed consortia -- Metagenomics -- Functional enzyme; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2022.118244
• Languages: English
• ISSNs: 0043-1354

Abstract

Highlights: 39–49% recovery in MP was obtained with well-constructed consortia bioaugmentation. Acetogenesis step was better accelerated with acclimatized consortia. Methanogenesis step was better accelerated with well-constructed consortia. Glycine hydroxymethyltransferase and phosphoglycerate mutase were found crucial. The increment of crucial enzymes could break through the bottleneck on enhancing MP. Abstract: Bioaugmentation is an attractive method to improve methane production (MP) in the anaerobic digestion (AD) process. In this study, to tackle the ammonia inhibition problem, a long-term (operating over 6 months) acclimatized consortia and a well-constructed consortia were selected as the bioaugmentation consortia for sequencing batch AD reactors fed with dairy manure and pig manure under mesophilic condition. Similar responses, in terms of the reactor performance and microorganisms structure to the different consortia, were observed with both manure kinds indicating that the effectiveness of bioaugmentation was mainly decided by the composition of the added consortia, not the feedstock. 39 – 49% increment in MP was obtained in the reactors bioaugmented with well-constructed consortia, which was higher than the acclimatized consortia (about 25% increment in MP). Both acetogenesis and methanogenesis (advantageous) steps were stimulated with well-constructed consortia bioaugmentation. According to key functional enzyme analysis, the increment of glycine hydroxymethyltransferase and phosphoglycerate mutase might be the critical point in the bioaugmented AD system. Based on the higher functional contribution rate of the well-constructed consortia bioaugmentation reactors, Methanosarcina could have expressed more comprehensive functions or performed stronger activities in different functions than Methanosaeta . Graphical abstract: Image, graphical abstract