Metagenomic analysis of microbial community structure and distribution of resistance genes in Daihai Lake, China. (1st June 2022)

• Record Type: Journal Article
• Authors: Du, Caili; Yang, Fang; Li, Xiaoguang; Liao, Haiqing; Li, Zhonghong; Gao, Jiayue; Zhang, Lieyu
• Is Part Of: Environmental pollution. Volume 302(2022)
• Publication Date: 2022-06-01
• Subjects: Metagenomic analysis -- Resistance gene -- Bacterial diversity -- Sediment -- Daihai lake; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.119065
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: The emergence of resistance genes is a global phenomenon that poses a significant threat to both animals and humans. Lakes are important reservoirs of genes that confer resistant to antibiotics and metals. In this study, we investigated the distribution and diversity of antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) in the sediment of Daihai Lake using high-throughput sequencing and metagenomic analysis. The results indicated that all sampling sites had similar bacterial community structures, with Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes being the most abundant. A total of 16 ARG types containing 111 ARG subtypes were deposited in the sediment. Among the resistance genes to bacitracin, multidrug, macrolide-lincosamide-streptogramin (MLS), tetracycline, beta-lactam, and sulfonamide were the dominant ARG types, accounting for 89.9–94.3% of the total ARGs. Additionally, 15 MRG types consisting of 146 MRG subtypes were identified. In all samples, MRGs of the same type presented resistance to Pb, Ni, Hg, W, Zn, Ag, Cr, Fe, As, Cu, and multimetals. Overall, the distribution and diversity of antibiotic and metal resistance genes showed no significant differences in the samples. Plasmids (91.03–91.82%) were the most dominant mobile genetic elements in the sediments of Daihai Lake. Network analysis indicated that the target ARGs and MRGs were significantly positively correlated with the microorganisms. Potential hosts for various ARGs and MRGs include Proteobacteria, Euryarchaeota, Actinobacteria, Chloroflexi, and Bacteroidetes. Graphical abstract: Image 1 Highlights: ARGs and MRGs in sediment from Daihai Lake were investigated. A total of 16 ARG types containing 111 ARG subtypes were deposited in the sediment. Distribution and components of ARGs and MRGs showed no significant differences. ARGs and MRGs were significantly positively correlated with microorganisms.