A non-waste strategy for enzymatic hydrolysis of cellulose recovered from domestic wastewater. Issue 10 (29th April 2022)

• Record Type: Journal Article
• Authors: Libardi, Nelson; Vandenberghe, Luciana Porto de Souza; Vásquez, Zulma Sarmiento; Tanobe, Valcineide; Carvalho, Júlio César de; Soccol, Carlos Ricardo
• Is Part Of: Environmental technology. Volume 43:Issue 10(2022)
• Page Start: 1503
• Page End: 1512
• Publication Date: 2022-04-29
• Subjects: Cellulose -- resource recovery -- enzymatic degradation -- residue valorization; Environmental engineering -- Periodicals; Environmental protection -- Periodicals; 628.05
• DOI: 10.1080/09593330.2020.1840635
• Languages: English
• ISSNs: 0959-3330

Abstract

ABSTRACT: Cellulose is a potential resource to be recovered from wastewater treatment plants (WWTP). Enzyme formulations can be employed to hydrolyze cellulose into fermentable sugars, to be further used as biochemical building blocks or reducing its recalcitrance to further treatment processes. This study proposed the production, recovery and formulation of cellulase using domestic wastewater as culture medium and its application for the hydrolysis of cellulosic residues recovered from WWTPs. Cellulose was recovered from raw sanitary wastewater using a fine-mesh sieve (0.35 mm) and quantified through enzymatic hydrolysis and thermogravimetric analysis. The production, concentration and formulation of cellulase enzyme resulted in an enzymatic blend of endoglucanases (7.3 UFP /mL), cellobiohydrolases (7.4 UCMC /mL) and beta-glucosidases (4.4 UBGL /mL). The content of the recovered cellulosic material was 21.3% according to enzymatic hydrolysis and 27.7 for thermogravimetric results. The enzymatic hydrolysis of the WWTP residue using the produced cellulase (107.6 ± 10.2 mgreduc /gresidue ) showed better results than using the commercial cellulase complex (66.4 ± 2.5 mgreduc /gresidue ). This fact showed the potential of application of the produced enzyme for the hydrolysis of cellulosic residues recovered from WWTP processes. In a non-waste biorefinery approach, the generated hydrolysate can be further used for producing added-value biomolecules including biofuels and biochemicals. GRAPHICAL ABSTRACT: UF0001