Indocyanine green-loaded exosomes for image-guided glioma nano-therapy. Issue 1 (31st December 2022)

• Record Type: Journal Article
• Authors: Fan, Bo; Yang, Song; Wang, Yuan-yu; Zhang, Chao; Yang, Ji-peng; Wang, Li-qun; Lv, Zhong-qiang; Shi, Xue-fang; Fan, Zhen-zeng; Yang, Jian-kai
• Is Part Of: Journal of experimental nanoscience. Volume 17:Issue 1(2022)
• Page Start: 187
• Page End: 196
• Publication Date: 2022-12-31
• Subjects: RGE -- exosomes -- GBM -- ICG -- mice; Nanoscience -- Periodicals; Nanotechnology -- Periodicals; Nanomedicine -- Periodicals; Nanotechnology -- Periodicals; 620.5
• DOI: 10.1080/17458080.2022.2060500
• Languages: English
• ISSNs: 1745-8080

Abstract

Abstract: Glioblastoma multiforme (GBM) is the most aggressive and deadliest type of brain tumor with a > >th he most aggressive and deadliest type of brain tumor witnear-infrared fluorescent dye, but it is hard to directly target brain tumor. Therefore, our study aimed to develop a new carrier for ICG to accurately visualize cancer tissue during surgery. Exosomes were isolated from culture U-87 MG cells, then loaded with Indocyanine green (ICG) and conjugated with RGERPPR peptide (RGE) (R-exo-ICG). The parameters of R-exo-ICG were analyzed and injected into tumor-bearing nude mice to evaluate its anti-tumor effect. R-exo-ICGs were nano size, and completely released ICG within 24 h. RGE modification increased cell uptake of exosomes, and induced cell apoptosis through activating Caspase 3 signaling pathway after laser irradiation. Moreover, R-exo-ICG was largely accumulated in the brain tumor in vivo, and inhibited tumor growth in tumor-bearing mice. RGE-conjugated exosomes that loaded ICG could target brain tumor in mice and significantly inhibited tumor growth in mice, which suggested that R-exo-ICG might serve as a novel approach for GBM treatment.