Development of a sensitive chicken IgY-based enzyme-linked immunosorbent assay for detection of mebendazole in pork and mutton. Issue 1 (29th March 2022)

• Record Type: Journal Article
• Authors: Zhu, Mi; Tang, Fang; Huo, Nairui; He, Jinxin; Gu, Shaopeng
• Is Part Of: Journal of environmental science and health. Volume 57:Issue 1(2022)
• Page Start: 47
• Page End: 53
• Publication Date: 2022-03-29
• Subjects: Mebendazole -- chicken IgY -- ELISA -- pork -- mutton; Pesticides -- Environmental aspects -- Periodicals; Food contamination -- Periodicals; Agricultural wastes -- Environmental aspects -- Periodicals; Ecology -- periodicals; Pesticides -- toxicity -- periodicals; Food Contamination -- periodicals; 363.73
• DOI: 10.1080/03601234.2021.2022944
• Languages: English
• ISSNs: 0360-1234

Abstract

Abstract: Chicken egg yolk IgY has proven to be qualified for analysis of targets in immunoassays. In order to explore the feasibility of chicken IgY-based ELISA for detection of mebendazole (MEB), the chicken IgY against MEB was generated in the laying hens. An enzyme-linked immunosorbent assay (ELISA) based on chicken IgY was developed for detection of MEB with a half-maximum signal inhibition concentration (IC50 ) of 3.65 ng mL −1 and a limit of detection of 0.25 ng mL −1 . The assay showed a lower cross reactivity (less than 1%) with other structures analogues (except amino-MEB with the values of 70.7%). The average recoveries of MEB spiked in pork and mutton muscle samples ranged from 93.6% to 106.3% with relative standard deviation less than 8.78% and 10.85% for intra-assay and inter-assay, respectively, and agreed well with those of high-performance liquid chromatography. Our results indicate that generated IgY could be used as a robust reagent for routine screening analysis of small molecular compounds residues in food samples.