Environmental carbon tetrachloride exposure disrupts the liver structure and metabolic detoxification function in mice via p38MAPK/NF-κB/NLRP3 pathway. Issue 1 (31st December 2022)

• Record Type: Journal Article
• Authors: Wei, Yuanyuan; Ma, Danyang; Fan, Yimeng; Gao, Chen; Wang, Qingtao; Yuan, Yanyan; Zhang, Yannan; Han, Juncheng; Hao, Zhihui
• Is Part Of: Food and agricultural immunology. Volume 33:Issue 1(2022)
• Page Start: 235
• Page End: 251
• Publication Date: 2022-12-31
• Subjects: Carbon tetrachloride -- liver injury -- antioxidant -- inflammation -- network toxicology; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2022.2060192
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: We investigated the effects of carbon tetrachloride (CCl4 ) exposure on liver detoxification. We confirmed that 0.2%-0.8% CCl4 decreased the liver carboxylesterase (CarE), lactate dehydrogenase (LDH) activity and total protein (TP) levels, while 0.2% and 0.8% CCl4 increased the liver acetylcholine esterase (AChE) activity ( P < 0.05). It was observed0.4%-0.8% CCl4 increased serum glutathione S-transferase (GST) and catalase (CAT) activity, 0.1%-0.8% CCl4 increased the level of malondialdehyde (MDA), and 0.2% CCl4 increased the level of total superoxide dismutase (T-SOD). The expression of nod-like receptor protein 3 (NLRP3), interleukin-1β (IL-1β), and tumour necrosis factor-α (TNF-α) were significantly elevated in 0.2%-0.8% CCl4 exposure ( P < 0.05). The expression of p38MAPK, gasdermin D (GSDMD), and nuclear factor kappa-B kinase (IKK) was decreased in 0.1%-0.8% CCl4 exposure, while the apoptosis was not statistically different in all groups. This indicates that 0.1% CCl4 exposure could damage the liver structure and detoxification function via p38MAPK/NF-κB/NLRP3 pathway. GRAPHICAL ABSTRACT: UF0001