A method for simultaneously crosslinking and functionalizing extracellular matrix-based biomaterials as bioprosthetic heart valves with enhanced endothelialization and reduced inflammation. (1st January 2021)

• Record Type: Journal Article
• Authors: Guo, Gaoyang; Jin, Linhe; Wu, Binggang; He, Haiyang; Yang, Fan; Xu, Liangpeng; Lei, Yang; Wang, Yunbing
• Is Part Of: Acta biomaterialia. Volume 119(2021)
• Page Start: 89
• Page End: 100
• Publication Date: 2021-01-01
• Subjects: Hybrid valves -- Endothelialization -- Anti-inflammation -- Decellularized extracellular matrix; APS Ammonium Persulfate -- Arg-1 Arginase-1 -- ATR-FTIR Attenuated Total Reflection Flourier Transformed Infrared -- BHVs Bioprosthetic Heart Valves -- CCK-8 Cell Counting Kit-8 -- cDNA Complementary DNA -- DMEM Dulbecco's Modified Eagle Medium -- DS Degree of Substitution -- DSC Differential Scanning Calorimeter -- DW Deionized Water -- D-PP Decellularized PP -- ECM Extracellular Matrix -- EDS Energy Dispersive Spectrometer -- FBS Fetal Bovine Serum -- FITC-BSA FITC-Bovine Serum Albumin -- FITC-FBG FITC-Fibrinogen -- GLUT Glutaraldehyde -- GLUT-PP Glutaraldehyde-treated Porcine Pericardium -- HUVEC Human Umbilical Vein Endothelial Cell -- ICP-OES Inductively Coupled Plasma Optical Emission Spectrometer -- IL-1R1 Interleukin-1 Receptor 1 -- MA Methacrylic Anhydride -- MA-PP Methacrylated Porcine Pericardium -- MHVs Mechanical Heart Valves -- MMPs Matrix Metalloproteinase -- NO Nitric Oxide -- PBS Phosphate-Buffered Saline -- PMA-PP Polymerizing the MA-PP -- PP Porcine Pericardium -- PRP Platelet-Rich Plasma -- PSPM-PP Copolymerized MA-PP with SPM -- RBCs Red Blood Cells -- SD Rat Sprague-Dawley Rat -- SDS Sodium-Dodecyl Sulphate -- SEM Scanning Electron Microscope -- SHS Sodium Hydrogen Sulfite -- SMC Smooth Muscle Cells -- SPM 3-Sulfopropyl Methacrylate Potassium Salt -- TAVR Transcatheter Aortic Valve Replacement -- TNF-α Tumor Necrosis Factor-α; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2020.10.029
• Languages: English
• ISSNs: 1742-7061

Abstract

Abstract: With the coming of an aging society and the emergence of transcatheter valve technology, the implantation of bioprosthetic heart valves (BHVs) in patients with valvular disease has significantly increased worldwide. Currently, most clinically available BHVs are crosslinked with glutaraldehyde (GLUT). However, the GLUT treated BHV is less durable due to the combined effect of multiple factors such as cytotoxicity, immune responses, and calcification. In this study, the in-situ polymerization of sulfonic monomers with a decellularized extracellular matrix (ECM) was performed to simultaneously achieve the crosslinking and functionalization of ECM. Subsequently, the feasibility of the hybrid ECM used as leaflet material of BHV was evaluated. In in-vitro tests, the results indicated that the hybrid ECM fixed collagen efficiently and the introduction of sulfonic polymer promoted the proliferation and migration of human umbilical vein endothelial cells (HUVECs). In in-vivo tests, after being implanted in SD rats and mice, the hybrid ECM significantly inhibited immune response and calcification compared with the non-hybrid counterpart and GLUT crosslinked tissue. These results indicated that the hybrid ECM exhibited more competitive stability and better biocompatibility compared to these features in GLUT-crosslinked valve. Therefore, the sulfonic polymer hybrid ECM provides a potential material for more durable BHV and the in-situ polymerization strategy can serve as a general treatment method for tissue crosslinking as well as tailoring the biophysical properties of ECM.