Bacterial sensing and killing based on fluorescent vesicles formed by cationic Gemini surfactants and AIEgen probe. (May 2022)

• Record Type: Journal Article
• Authors: Zhang, Xinwei; Ma, Jiao; Ou, Kaide; Cong, Xin; Zhang, Binbin; Zhou, Qi; Liao, Yonggui; Yang, Yajiang; Wang, Hong
• Is Part Of: Dyes and pigments. Volume 201(2022)
• Publication Date: 2022-05
• Subjects: Bacterial sensing and killing -- Fluorescent vesicles -- Gemini surfactants -- AIEgen probe; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2022.110245
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: In this work, a novel type of vesicles (VT/G ) composed of Gemini cationic surfactant (G12-8-12 ) and TPE-2OH, an AIEgen fluorescence probe, were prepared by methanol evaporation method. Herein, G12-8-12 has good bacteriostatic function. TPE-2OH with aggregation-induced emission feature resided in the interlayer of VT/G . The average size of VT/G was about 1.2–1.4 μm measured by dynamic light scattering. Zeta potential studies indicated that VT/G could bind to bacteria like S. aureus and E. coli under electrostatic interaction, increasing the size of the complex of VT/G and bacteria to 4.5–6 μm. Because VT/G interlayer was compressed by external bacteria, the aggregation degree of TPE-2OH was increased, and the fluorescence intensity was increased by 3 times compared with VT/G alone. The linear increase in fluorescence intensity with the increase of bacteria content implied that it is possible to sense bacteria. After the bacteria were co-incubated with VT/G, almost no bacteria growth was observed with the light irradiation for 30 min, indicating that VT/G showed good bactericidal effect. This can be ascribed to the synergistic effect of the inherent bactericidal property of G12-8-12 and the oxygen-active substances generated from TPE-2OH. This new strategy integrating bacteria detection and killing showed good potential application. Graphical abstract: The vesicles composed of Gemini surfactant (G12-8-12 ) and AIEgen probe (TPE-2OH) is developed for bacteria sensing and killing based on the aggregation of TPE-2OH induced by bacteria and the synergistic bacteriostatic effect of G12-8-12 and ROS generated from TPE-2OH aggregates under light irradiation. Image 1 Highlights: The luminescent vesicle contained Gemini surfactant G12-8-12 and AIEgen (TPE-2OH) was prepared. The prepared vesicles blend sensing and killing of bacteria simultaneously. The synergistic bactericidal ability of G12-8-12 and ROS originated from TPE-2OH was realized.