Characterisation of cellulose-degrading organisms in an anaerobic digester. (May 2022)

• Record Type: Journal Article
• Authors: Struckmann Poulsen, Jan; de Jonge, Nadieh; Vieira Macêdo, Williane; Rask Dalby, Frederik; Feilberg, Anders; Lund Nielsen, Jeppe
• Is Part Of: Bioresource technology. Volume 351(2022)
• Publication Date: 2022-05
• Subjects: Protein stable isotope probing -- Lignocellulosic biomass -- Metaproteomics -- Microbial community -- Anaerobic digestion; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.126933
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Protein stable isotope probing revealed microbial function and identity. Corynebacterium was identified through CAZyme, and metagenomic analysis. Proteogenomics identified six genera associated with cellulose degradation. Microbial 13 C-cellulose assimilation yielded twenty 13 C-labelled peptides. Meta-omics provides insight into microbial degradation of cellulosic biomass. Abstract: The recalcitrant nature of lignocellulosic biomass hinders efficient exploitation of this fraction for energy production. A better understanding of the microorganisms able to convert plant-based feedstocks is needed to improve anaerobic digestion of lignocellulosic biomass. In this study, active thermophilic cellulose-degrading microorganisms were identified from a full-scale anaerobic digester fed with maize by using metagenome-resolved protein stable isotope probing (protein-SIP). 13 C-cellulose was converted into 13 C-methane with a 13/12 C isotope ratio of 0.127 after two days of incubation. Metagenomic analysis revealed 238 different genes coding for carbohydrate-active enzymes (CAZymes), six of which were directly associated with cellulose degradation. The protein-SIP analysis identified twenty heavily labelled peptides deriving from microorganisms actively assimilating labelled carbon from the degradation of 13 C-cellulose, highlighting several members of the order Clostridiales . Corynebacterium was identified through CAZyme screening, amplicon analysis, and in the metagenome giving a strong identification of being a cellulose degrader.