Differential expression of matrix metalloproteinases in the fallopian tube of women with Chlamydia trachomatis-associated tubal ectopic pregnancy. (April 2022)

• Record Type: Journal Article
• Authors: Pant, Shipra; Dimri, Astha; Arora, Renu; Siraj, Fouzia; Raisuddin, Sheikh; Rastogi, Sangita
• Is Part Of: Microbial pathogenesis. Volume 165(2022)
• Publication Date: 2022-04
• Subjects: Chlamydia trachomatis -- Ectopic pregnancy -- Matrix metalloproteinases -- Tissue inhibitors of matrix metalloproteinases -- Real time PCR; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2022.105468
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Chlamydia trachomatis is an established risk factor for ectopic pregnancy (EP) in fallopian tube (FT). Matrix metalloproteinases (MMPs) have potential role in disease pathogenesis, however, dysregulation of extracellular matrix by MMPs/TIMPs (tissue inhibitors of MMPs) in infection-associated EP remains unknown. The aim was to study the expression of MMP-2, -9, -14/TIMP-1, -2, -3 in C. trachomatis -positive tubal EP patients. The study comprised of 100 tubal EP (Group I) and 100 tubal ligation patients (Group II; controls) enrolled from Department of Obstetrics and Gynaecology, VMMC and Safdarjung hospital, New Delhi (India) for collection of FT. Detection of C. trachomatis MOMP was done by PCR while quantitative expression of MMPs/TIMPs was studied by real-time PCR. Data was statistically evaluated by Graphpad prism. Overall, C . trachomatis was found in 18/100 tubal EP patients. After ruling out Neisseria gonnorhoeae and Mycoplasma genitalium, Group I was divided into Group Ia ( C. trachomatis DNA-positive) and Group Ib ( C. trachomatis DNA-negative; internal controls). Significant upregulation of MMP-2, -9, -14 and downregulated TIMP-1, -2, -3 were found in Group Ia versus controls (Groups Ib/II) (p < 0.05). Fold-change in MMP was significantly higher in Group Ia versus controls ('p' < 0.05). Maximum 5.5-fold upregulation was found in MMP-2. It is apparent by molecular analysis that differential expression of MMPs/TIMPs, particularly enhanced MMP-2 leads to tubal EP in C. trachomatis DNA-positive women. Highlights: Significant upregulation of MMPs and downregulated TIMPs was found in the C. trachomatis -positive women with tubal EP. Fold-change in MMPs was significantly higher in C. trachomatis -positive women with tubal EP. Maximum 5.5-fold upregulation was found in MMP-2. Differential expression of MMPs/ TIMPs, particularly enhanced MMP-2 leads to tubal EP in C. trachomatis -positive women.