The effect of stromal vascular fraction and Platelet-Rich Plasma combination on basic Fibroblast Growth Factor serum level during anal trauma healing in a Wistar rat model. (April 2022)

• Record Type: Journal Article
• Authors: Sujana, Ricko Sadli; Sulmiati, ; Mariana, Nita; Josh, Fonny; Laidding, Sachraswaty Rachman; Zainuddin, Andi Alfian; Faruk, Muhammad
• Is Part Of: Annals of medicine and surgery. Volume 76(2022)
• Publication Date: 2022-04
• Subjects: Basic fibroblast growth factor -- Stromal vascular fraction -- Platelet-rich plasma -- Anal trauma -- Wound healing; Surgery -- Periodicals; Medicine -- Periodicals; General Surgery -- Periodicals; Education, Medical -- Periodicals; Periodicals; 617
• DOI: 10.1016/j.amsu.2022.103375
• Languages: English
• ISSNs: 2049-0801

Abstract

Abstract: Introduction: Stromal Vascular Fraction (SVF) and Platelet-Rich Plasma (PRP) application play important roles in the healing process by increasing basic Fibroblast Growth Factor (bFGF) secretion. This research assesses the effect of combined SVF and PRP local injection on bFGF levels, using an anal trauma model in Wistar rats. Method: Twenty-eight adult Wistar rats were divided into three groups. Groups A and B underwent modified surgical anal trauma and repair; Group A was treated with the SVF and PRP combination local injection, while Group B was treated with only normal saline. Subsequently, we examined bFGF levels in Groups A and B on days 1, 7, and 14. Group C consisted of healthy controls sacrificed on day 0 to obtain baseline data on bFGF levels. Results: The bFGF levels were higher in Group A than in Group B on every experimental day. The Repeated Measures test shows a significant increase in bFGF levels on day 1 (p = 0.000), day 7 (p = 0.000), and day 14 (p = 0.000). This test also indicates that the local injection combination of SVF and PRP increased bFGF levels by 96.2% compared to the placebo group. Conclusion: The combination of SVF and PRP can increase bFGF levels during anal trauma healing in the Wistar rat model. Basic FGF is an important factor throughout the anal trauma healing process. Highlights: The combination of SVFs and PRP is effective to increase the concentration of bFGF. Administration of SVFS + PRP by injection was better at increasing the expression of bFGF than by placebo. The combination of SVFs and PRP increases the level of bFGF in the healing process of anal trauma.